Xcell-Center

Uncategorized

Successful Function of Autologous iPSC-Derived Dopamine Neurons following Transplantation in a Non-Human Primate Model of Parkinson’s Disease

http://www.cell.com/cell-stem-cell/abstract/S1934-5909(15)00056-9?rss=yes by

 

To view the full text, please login as a subscribed user or purchase a subscription. Click here to view the full text on ScienceDirect.

Figure 1

Functional Improvement of PD Motor Symptoms, Increased Dopamine Reuptake, and Reinnervation of the Transplanted Putamen after Autologous Transplantation of CM iPSC-Derived Dopamine Neurons

(A) Differentiated CM-iPSCs were transplanted unilaterally into the putamen of three CMs with stable, bilateral parkinsonism (MF25-04, MF66-02, MF27-04). The animals were followed for 1–2 years after transplantation. From 6 months after transplantation, functional improvement was observed in MF25-04, as determined by a sustained increase in global daytime (6 a.m. to 6 p.m.) activity. The non-transplanted control group and non-surviving transplant group represent the average data of n = 4 and n = 3 animals, respectively, and error bars show the SEM.

(B) Fine-motor skills in MF25-04 were assessed using a computerized reaching task MAP. At 2 years after transplantation, MAP performance in the left upper limb was significantly improved compared with pretransplantation values (p < 0.05, one-way ANOVA followed by Tukey’s multiple comparison test). No change in performance was observed in the right upper limb. Data shown represent averages of five repeated tests (baseline), two repeated tests (1 year after transplantation), and three repeated tests (2 years after transplantation). Error bars represent the SEM.

(C) Functional analysis of dopamine reuptake in MF25-04 was measured by PET neuroimaging for 11C-CFT, a marker of the DAT. Increased 11C-CFT binding was observed in the transplanted putamen at 2 years after transplantation. White arrows indicate areas of hyperintense CFT PET signal.

(D) Low-power photomicrograph of DAT immunostaining in the transplanted (right, R) and non-transplanted (left, L) putamen in MF25-04 shows reinnervation of the transplanted side. Deposits of grafted dopamine neurons are indicated with boxes (g). IC, internal capsule; LGP, lateral globus pallidus; LV, lateral ventricle; 3V, third ventricle; cc, corpus callosum.

(E) Grafted dopamine neurons were also labeled using tyrosine hydroxylase (TH). The boxed area is shown at higher magnification in the right. Robust survival of dopamine neurons with outgrowth integration into the host putamen was observed.

Figure 2

Phenotypes of Engrafted CM iPSC-Derived Neurons at 2 Years after Autologous Transplantation

Postmortem immunohistochemical analysis was used to further characterize the graft in MF25-04.

(A) Immunofluorescence staining confirmed that transplanted dopamine neurons were colabeled for FOXA2, TH, and βIII tubulin at 2 years after transplantation.

(B) Labeling for DAT, TH, and TOM20 (a mitochondrial outer membrane protein) showed a punctate expression of DAT along the fibers of transplanted dopamine neurons and a typical localization of mitochondria throughout the cell soma and neurites.

(C) Immunofluorescence staining for 5-HT demonstrated the presence and localization of serotonergic neurons within the graft.

(D) Labeling for DARPP-32, a marker of striatal GABAergic medium spiny neurons, shows robust labeling in the host putamen and occasional DARPP-32-ir fibers at the graft-host border.

(E and F) Ki-67 was used to determine whether proliferating cells were present in the CM-iPSC-derived neural cell graft from MF25-04. No Ki-67-immunoreactive cells were observed in the graft (E). As a positive control, several Ki-67-immunoreactive proliferating cells were observed in the hippocampus of the same animal (F) (identified with arrows).

(G and H) Histological analysis of microglia using Iba1 in the host putamen (G) and graft (H) shows typical resting microglia.

Highlights

  • A non-human primate model tests cell transplantation for PD therapy
  • Autologous iPSC dopamine neurons can provide long-term functional recovery
  • Transplanted cells survive for up to 2 years and reinnervate the host brain

Summary

Autologous transplantation of patient-specific induced pluripotent stem cell (iPSC)-derived neurons is a potential clinical approach for treatment of neurological disease. Preclinical demonstration of long-term efficacy, feasibility, and safety of iPSC-derived dopamine neurons in non-human primate models will be an important step in clinical development of cell therapy. Here, we analyzed cynomolgus monkey (CM) iPSC-derived midbrain dopamine neurons for up to 2 years following autologous transplantation in a Parkinson’s disease (PD) model. In one animal, with the most successful protocol, we found that unilateral engraftment of CM-iPSCs could provide a gradual onset of functional motor improvement contralateral to the side of dopamine neuron transplantation, and increased motor activity, without a need for immunosuppression. Postmortem analyses demonstrated robust survival of midbrain-like dopaminergic neurons and extensive outgrowth into the transplanted putamen. Our proof of concept findings support further development of autologous iPSC-derived cell transplantation for treatment of PD.

To access this article, please choose from the options below

Register an Account

If you do not have an account, create one by clicking the button below, and take full advantage of this site’s features.